09-15 (2) Effect of Xiaochaihu Decoction on Cytokines and MAO in Experimental Autoimmune Prostatitis Rats

Abstract

Objective To observe the effect of Xiaochaihu Decoction on cytokines and MAO in model rats with experimental autoimmune prostatitis (EAP) to provide an experimental basis for the use of Chinese herbal prescriptions in the treatment of chronic prostatitis. Methods Thirty male Wistar rats were randomly divided into blank Group, model group and treatment group (Xiaochaihu Decoction). Except the blank group, rats in model and treatment groups were injected subcutaneously in multiple points on days 0 and 30 with prostatic protein extractive solution (60 mg/mL), and intraperitoneally injected with diph-theria-pertussis and tetanus vaccine (DPT vaccine) to establish the EAP model. Model rats were administrated Xiaochaihu Decoction and were sacrificed after 4 weeks. Pathological changes in the prostate gland were observed with HE staining and changes in serum interleukin-6 (IL-6), interleukin-8 (IL-8), and Tumor necrosis factor-α (TNF-α) levels were detected with enzyme-linked immunosorbent assay (ELISA). Quantitative PCR to observe the expression of IL-17, CCL2 and MAOA/B. Results After treatment with Xiaochaihu Decoction, the serum IL-6, IL-8, and TNF-α levels all significantly lower in the treatment group as compared with the model group (P < 0.05). The intervention of Xiaochaihu Decoction can change the state of abnormally elevated IL-17, CCL2 and MAOA expression levels. Conclusions The therapeutic efficacy remarkable from the theory of heating into the sperm chamber to experimental autoimmune prostatitis rats.