V3N1-01 The Temporal Profile of OPG Expression and Regulatory Role on Ischemic Brain Injury in Rats After MCAo

Abstract

Objective To investigate the temporal profile of osteoprotegerin (OPG) in middle cerebral artery occlusion (MCAo) rats and the serum level of RANKL and ALP in OPG deficient and wild type rats. Methods Rats was anesthetized and subjected to MCAo by transient occlusion of middle cerebral artery occlusion. The serum level of OPG, RANKL and ALP in rats after MCAo was examined by ELISA assay. The protein expression of OPG in ischemic brain was determined by Western blot analysis. Results The level of OPG in the rat serum was significantly increased from 6 h after MCAo and peaked at 12-24 and 72-168 h. The protein expression of OPG was upregulated from 12 h after MCAo and peaked at 72 h. The level of RANKL and ALP was significantly decreased in OPG deficient rats after MCAo. Conclusion OPG/RANKL signaling is associated with brain injury after MCAo, indicating a potential therapeutic target for ischemic stroke.